GEL FILTRATION PROPERTIES OF CCD-PREPARED E. COLI B sRNA

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Gel Filtration Properties of Ccd-prepared E. Coli B Srna.

1 Isaacs, A., and J. Lindenmann, Proc. Roy. Soc. (London), B147, 258 (1957). 2 Rotem, Z., R. A. Cox, and A. Isaacs, Nature, 197, 564 (1963). 3Abbreviations: RNA and DNA, ribonucleic acid and deoxyribonucleic acid; RNAase, pancreatic ribonuclease; DNAase, pancreatic deoxyribonuclease; CE cells, chick embryo cells. 4O'Dell, T. B., H. N. Wright, and R. N. Bieter, J. Pharmacol. Exptl. Therap., 107,...

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Countercurrent Distribution Studies of E. Coli B Srna.

Since Holley'sl 2 initial application of countercurrent distribution (CCD) to the problem of the separation of the soluble ribonucleic acids (sRNA) of rat liver and yeast, considerable work has been done in this area by several investigators employing this technique.3-'0 Their primary interest has been to obtain purified specific sRNA's for structural studies. In the course of this work Doctor ...

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Two interconvertible forms of tryptophanyl sRNA in E. coli.

8 Felsenfeld, G., and S. Huang, Biochim. Biophys. Acta, 51, 19 (1961). 9 Lyons, J. W., and L. Kotin, J. Am. Chem. Soc., 86, 3634 (1964). 10 Boedtker, H., J. Mol. Biol., 2, 171 (1960). 11 Monier, R., and M. Grunberg-Manago, in Acides Ribonucleiques et Polyphosphates, Colloques Internationaux de Centre National du la Recherche Scientifique no. 106 (Paris, 1962), p. 163. 12 Giacomoni, D., and S. S...

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sRNA-Mediated Control of Transcription Termination in E. coli

Bacterial small RNAs (sRNAs) have been implicated in various aspects of post-transcriptional gene regulation. Here, we demonstrate that sRNAs also act at the level of transcription termination. We use the rpoS gene, which encodes a general stress sigma factor σ(S), as a model system, and show that sRNAs DsrA, ArcZ, and RprA bind the rpoS 5'UTR to suppress premature Rho-dependent transcription t...

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Gel filtration of sialoglycoproteins.

The role of sialic acid in the gel-filtration behaviour of sialoglycoproteins was investigated by using the separated isoenzymes of purified human liver alpha-L-fucosidase and several other well-known sialic acid-containing glycoproteins (fetuin, alpha1-acid glycoprotein, thyroglobulin and bovine submaxillary mucin). For each glycoprotein studied, gel filtration of its desialylated derivative g...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1964

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.52.3.744